Many of the effects of hypoxia on differentiation, cell cycle arrest, cancer metastasis and apoptosis are mediated primarily through the direct actions of transcription factors like HIF-1a, DEC1 and p53. I am using chromatin immunoprecipitation and other biochemical techniques to study the mechanisms of specific gene phenomena and to discover previously unidentified promoter targets. This approach is being used to study both the regulation of gene expression by p53 during hypoxic stress and the regulation of adipogenesis by hypoxia and steroid hormones.
Sutphin, P. D., Chan, D.A., Li, J.M., Turcotte, S., Krieg, A.J., and Giaccia, A.J. (2007). Targeting the loss of the von Hippel-Lindau tumor suppressor gene in renal cell carcinoma cells. Cancer Res. 67(12):5896-905.
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Krieg A. J., Hammond E. M., and Giaccia A. J. Functional analysis of p53 binding during differential stresses. Mol Cell Biol 2006 Oct; 26(29):7030-45.
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Freiberg R. A., Krieg, A. J., Giaccia, A. J., and Hammond, E. M. Checking in on hypoxia/reoxygenation. Cell Cycle. 2006 Jun;5(12):1304-7.
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Hammond, E. M., Mandell, D. J., Salim, A., Krieg, A. J., Johnson, T. M., Shirazi, H. A., Attardi, L. D., and Giaccia, A. J. Genome-Wide Analysis of p53 under Hypoxic Conditions. Mol Cell Biol. 2006; 26 (9) 3492-504.
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